广西医学

目的探讨胃癌组织内质网核糖体结合蛋白1（RRBP1）、层粘连蛋白亚基β3（LAMB3）的mRNA表达水平及其与患者临床病理特征、预后的关系。方法收集373例胃癌患者的胃癌组织标本和癌旁组织标本。采用实时荧光定量PCR检测胃癌组织和癌旁组织中RRBP1和LAMB3的mRNA表达水平，分析RRBP1和LAMB3的mRNA表达水平与胃癌患者临床病理特征的关系，绘制生存曲线分析胃癌组织中RRBP1和LAMB3的mRNA表达水平与患者预后的关系，采用COX回归模型分析胃癌患者预后的影响因素。结果与癌旁组织相比，胃癌组织中RRBP1和LAMB3的mRNA表达水平升高（P＜0.05）。肿瘤直径≥4 cm、分化程度为低或未分化、浸润深度为T3～T4、发生淋巴结转移及远处转移的胃癌患者RRBP1 mRNA表达水平更高（P＜0.05）；分化程度为低或未分化、浸润深度为T3～T4、发生淋巴结转移及远处转移的胃癌患者LAMB3 mRNA表达水平更高（P＜0.05）。RRBP1高表达组和LAMB3高表达组的3年生存率分别低于RRBP1低表达组和LAMB3低表达组（P＜0.05）。COX回归分析结果显示，RRBP1 mRNA高表达、LAMB3 mRNA高表达、低或未分化程度、T3～T4浸润深度和发生淋巴结转移均是影响胃癌患者预后的独立危险因素（P＜0.05）。结论RRBP1和LAMB3 mRNA在胃癌组织中高表达，且与患者临床病理特征和预后密切相关。

ObjectiveTo investigate the mRNA expressions of endoplasmic reticulum ribosome-binding protein 1 (RRBP1) and laminin subunit beta 3 (LAMB3) in gastric cancerous tissues and their relations with clinicopathological features and prognosis in patients. MethodsSpecimens of gastric cancerous and paracancerous tissues among 373 patients with gastric cancer were collected. The mRNA expressions of RRBP1 and LAMB3 in gastric cancerous and paracancerous tissues were detected by employing the real-time fluorescence quantitative PCR. The relation of RRBP1 and LAMB3 mRNA expressions with clinicopathological features of patients with gastric cancer was analyzed. The survival curve was drawn to analyze the relation of RRBP1 and LAMB3 mRNA expressions in gastric cancerous tissues with prognosis of patients. The COX regression model was used to analyze the influencing factors for prognosis of patients with gastric cancer. ResultsCompared with paracancerous tissues, the mRNA expressions of RRBP1 and LAMB3 in gastric cancerous tissues were elevated (P＜0.05). Gastric cancer patients with tumor diameter≥4 cm, low- or undifferentiated differentiation degree, invasion depth in T3-T4, the occurrence of lymph node metastasis and distant metastasis obtained a higher mRNA expression of RRBP1 (P＜0.05); moreover, gastric cancer patients with low- or undifferentiated differentiation degree, invasion depth in T3-T4, the occurrence of lymph node metastasis and distant metastasis obtained a higher mRNA expression of LAMB3 (P＜0.05). The three-year survival rates of the RRBP1 high expression group and the LAMB3 high expression group were lower than those of the RRBP1 low expression group and the LAMB3 low expression group (P＜0.05). The results of COX regression analysis revealed that high expressions of RRBP1 and LAMB3 mRNA, low- or undifferentiated differentiation degree, invasion depth in T3-T4, and the occurrence of lymph node metastasis were the independent risk factors for affecting prognosis of patients with gastric cancer (P＜0.05).ConclusionRRBP1 and LAMB3 mRNA are highly expressed in gastric cancerous tissues, and are closely related to clinicopathological features and prognosis of patients.