ObjectiveTo explore the mechanism of Jiejing Sanyu Decoction for the treatment of rotator cuff tear by utilizing network pharmacology and molecular docking technique. Methods(1) The main active components of 10 Traditional Chinese Medicines in Jiejing Sanyu Decoction were screened through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) and SwissADME platform, and then the effect targets of main active components were screened through the TCMSP and SwissTargetPrediction platform. Targets related to rotator cuff tear were obtained by GeneCards and OMIM databases, and then was intersected with the effect targets of main active components for acquiring potential effect targets of Jiejing Sanyu Decoction for treating rotator cuff tear. (2) The component-target network was established by using the Cytoscape 3.9.1 software for screening the key active components of Jiejing Sanyu Decoction for treating rotator cuff tear. The protein-protein interaction network of potential effect targets was established for screening core targets of Jiejing Sanyu Decoction for treating rotator cuff tear through the STRING 11.5 platform and Cytoscape 3.9.1 software. (3) The Gene Ontology (GO) functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were conducted on potential effect targets by employing DAVID database. (4) The AutoDock 1.5.6 software was used to perform molecular docking between key active components and proteins of the core targets. Results(1) A total of 835 effect targets of main active components of Jiejing Sanyu Decoction, 357 targets related to rotator cuff tear were obtained, and 95 potential effect targets were acquired after intersection. (2) A total of 5 key active components and 5 core targets were obtained, the former contained quercetin, cyclo (L-phe-L-pro), luteolin, L-prolyl-L-leucyl-L-leucylglycine, and kaempferol, and the latter included interleukin (IL)-1B, serine/threonine kinase 1 (AKT1), vascular endothelial growth factor A (VEGFA), matrix metalloproteinase 9 (MMP9), and fibronectin 1 (FN1). (3) The results of GO functional enrichment analysis revealed that the biological processes involved in potential effect targets were mainly response to lipopolysaccharide, response to hypoxia, response to hydrogen peroxide, collagen catabolic process, proteolysis, etc.; in addition, the results of KEGG pathway enrichment analysis revealed that the potential targets are mainly involved in advanced glycation end products and receptor of advanced glycation end products signaling pathway, hypoxia-inducible factor 1 signaling pathway, tumor necrosis factor signaling pathway, IL-17 signaling pathway, etc. (4) The results of molecular docking depicted that all the key active components exerted good binding activities with the core target proteins. ConclusionMultiple active components of Jiejing Sanyu Decoction may affect signaling pathways related to inflammatory responses, cell apoptosis, and angiogenesis, and further inhibit inflammatory responses, regulate local cell apoptosis in traumatic tissues, inhibit tissue excessive remodeling, so as to exert effects for treating rotator cuff tear through acting on IL-1B, AKT1, VEGFA, MMP9, FN1, and other targets.

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