广西医学

目的应用网络药理学方法探讨鹤兰墨香浴里精油平衡洗液外治银屑病的作用机制。方法鹤兰墨香浴里精油平衡洗液的主要成分为皂荚，利用中药系统药理学数据库与分析平台筛选皂荚的活性化学成分及其对应的作用靶点，利用GeneCards数据库、OMIM数据库检索银屑病的相关靶点，使用Venny 2.1.0平台获取皂荚活性化学成分作用靶点与银屑病相关靶点的交集靶点。针对皂荚治疗银屑病的相关靶点，利用Cytoscape 3.8.2软件构建皂荚活性化学成分-银屑病疾病靶点网络图，利用STRING数据库构建蛋白-蛋白相互作用(PPI)网络，并进行基因本体论（GO）功能富集分析和京都基因与基因组百科全书（KEGG）通路富集分析。结果获得皂荚的活性化学成分5个，分别是豆甾醇、谷甾醇、豆甾-4-烯-3,6-二酮、表儿茶素、廿碳-11-烯酸，其对应的作用靶点共162个。获得银屑病的相关靶点共4 104个，将皂荚活性化学成分作用靶点与银屑病相关靶点取交集，获得交集靶点106个。皂荚活性化学成分-银屑病疾病靶点网络图显示，表儿茶素的度值最大，其在皂荚治疗银屑病中发挥最重要的作用。PPI网络拓扑学分析结果显示，皂荚外治银屑病的关键靶点为VEGFA、AKT1、TNF、IL-6、MMP9、PTGS2、IL-1β、JUN、EGF、Caspase-3。富集分析结果显示，皂荚治疗银屑病涉及对无机物质的反应、对激素的反应、细胞群增殖的负调控、细胞迁移的正调控等生物过程，膜筏、膜微区、转录调节复合物、囊腔等细胞组分，DNA结合和转录因子结合、转录因子结合、RNA聚合酶Ⅱ特异性DNA结合和转录因子结合、蛋白质同源二聚活性等分子功能，TNF信号通路、IL-17信号通路等信号通路。结论鹤兰墨香浴里精油平衡洗液的主要成分皂荚可能通过表儿茶素、豆甾醇、豆甾-4-烯-3,6-二酮、廿碳-11-烯酸、谷甾醇等活性化学成分，作用于VEGFA、AKT1、TNF、IL-6、MMP9等相关靶点，影响IL-17、TNF等信号通路，从而达到治疗银屑病的目的。

ObjectiveTo explore the mechanism of Herllamo Bathart Essential Oil Shower Gel for the external treatment of psoriasis by employing the network pharmacology method.MethodsThe main component of Herllamo Bathart Essential Oil Shower Gel was Gleditsia sinensis, and the active chemical components and its corresponding effect targets of Gleditsia sinensis were screened by using the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform. Targets related to psoriasis were retrieved by employing the GeneCards and OMIM databases. The Venny 2.1.0 platform was used to obtain intersection targets between active chemical components of Gleditsia sinensis and targets related to psoriasis. For targets related to Gleditsia sinensis for treating psoriasis, the Cytoscape 3.8.2 software was used to establish network diagram of active chemical components of Gleditsia sinensis-disease targets of psoriasis, and protein-protein interaction (PPI) network was established by the STRING database, as well as the Gene Ontology (GO) functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed. ResultsA total of 5 active chemical components from Gleditsia sinensis, including stigmasterol, sitosterol, stigmast-4-ene-3,6-dione, epicatechin, and eicosanoid-11-enoic acid, were obtained, and there were 162 corresponding effect targets in total. There were 4104 targets related to psoriasis in total, and 106 intersection targets were obtained after acquiring intersection between active chemical components of Gleditsia sinensis and targets related to psoriasis. The network diagram of active chemical components of Gleditsia sinensis-disease targets of psoriasis revealed that epicatechin had the highest degree value, and it played the most important role in the treatment of psoriasis with Gleditsia sinensis. The results of PPI network topology analysis indicated that the key targets of Gleditsia sinensis for the external treatment of psoriasis were VEGFA, AKT1, TNF, IL-6, MMP9, PTGS2, IL-1β, JUN, EGF, and Caspase-3. The results of enrichment analysis indicated that Gleditsia sinensis for the treatment of psoriasis involved biological processes in terms of response to inorganic substances, response to hormones, negative regulation of cell mass proliferation, positive regulation of cell migration, etc.,involved cellular compositions with respect to membrane raft, membrane microdomain, transcriptional regulatory complex, and cystic cavity, etc.,and involved molecular functions of DNA binding and transcription factor binding, transcription factor binding, RNA polymerase II specific DNA binding and transcription factor binding, protein homologous dimerization activity, etc., as well as involved signaling pathways in terms of TNF signaling pathway, IL-17 signaling pathway, etc. ConclusionThe main component of Gleditsia sinensis from Herllamo Bathart Essential Oil Shower Gel may act on VEGFA, AKT1, TNF, IL-6, MMP9, and other relevant targets, affect IL-17, TNF, and other signaling pathways, so as to achieve the purpose of treatment of psoriasis by active chemical components in terms of epicatechin, stigmasterol, stigmast-4-ene-3,6-dione, eicosanoid-11-enoic acid, and sitosterol, etc.