Objective To explore the effect of Angelica sinensis polysaccharide on osteogenic differentiation potential of bone marrow stem cell (BMSC) in hypoxia. Methods BMSC was randomly divided into blank group, hypoxia group, or Angelica sinensis polysaccharide group. OriCellTM complete medium of adult BMSC osteogenesis⁃induced differentiation was used to perform culture in cells of the three groups, therein medium of the Angelica sinensis polysaccharide group contained 50 μg/mL Angelica sinensis polysaccharide. BMSC routine culture was given to the blank group (21% oxygen concentration), and BMSC of the hypoxia group and the Angelica sinensis polysaccharide group was placed in an environment with 6% oxygen concentration to perform culture. After 14 days of culture, BMSC morphological change and calcium salt deposition condition were observed in the three groups. BMSC osteogenic differentiation⁃associated proteins (osteopontin and osteocalcin) expressions, and BMSC osteogenic differentiation key transcription factor Runt⁃related transcription factor 2 (RUNX2) expression were detected in the three groups. Results  Compared with the blank group, the hypoxia group exhibited decreased expressions of osteocalcin, osteopontin, and RUNX2 (P<0.05), and presented as reduced high⁃density calcium nodules and calcium salt deposition. Compared with the hypoxia group, the Angelica sinensis polysaccharide group yielded elevated BMSC osteocalcin, osteopontin, and RUNX2 expressions (P<0.05), and presented as increased high⁃density calcium nodules and calcium salt deposition. Conclusion Hypoxia can inhibit BMSC osteogenic differentiation potential, and Angelica sinensis polysaccharide can effectively maintain stability of BMSC osteogenic differentiation potential in hypoxia.

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