Objective To explore the application effect of oblique incision approach via supraclavicular fossa in bilateral thyroid surgery. Methods The clinical data of 94 patients who underwent bilateral thyroid surgery were retrospectively analyzed, and patients were divided into oblique incision group (oblique incision approach via supraclavicular fossa, n=32), median incision group (incision approach via cervical white line, n=32), or endoscopic areola group (incision approach via endoscopic whole areola, n=30) according to the surgical approaches. Pain Visual Analogue Scale (VAS) and Numerical Scoring System (NSS) scale were employed to evaluate postoperative pain and incision satisfaction of patients, respectively. Perioperative indices in terms of operation duration, intraoperative bleeding volume, 24⁃hour postoperative drainage volume, and postoperative extubation time, postoperative pathologic findings, postoperative occurrence states of complications, postoperative subjective discomfort (incision pain, skin numbness in surgical area, swallowing discomfort), and postoperative pain VAS score and NSS scale score were compared between patients of the three groups. Results The operation duration was increased successively in the oblique incision group, median incision group and the endoscopic areola group (P<0.05). The oblique incision group exhibited less intraoperative bleeding volume and 24⁃hour postoperative drainage volume as compared with the median incision and endoscopic areola groups (P<0.05). There was no statistically significant difference in postoperative extubation time, the maximum diameter of lesion, number of central lymph nodes dissection, and metastasis rate of lymph nodes between the three groups (P>0.05). VAS score for pain 24 hours after operation was higher in the endoscopic areola group than in the oblique incision and the median incision groups (P<0.05). There was no statistically significant difference in postoperative incidence rate of complications, postoperative incidence rate of subjective discomfort, and 48⁃hour postoperative VAS score for pain between the three groups (P>0.05). Postoperative NSS scale score was higher in the oblique incision group and the endoscopic areola group than in the median incision group (P<0.05). Conclusion Application effect of oblique incision approach via supraclavicular fossa in bilateral thyroid surgery is favorable. Compared with incision approaches via cervical white line and endoscopic whole areola, it exhibits shorter operation duration, less intraoperative bleeding volume and postoperative drainage volume, and favorably relieved postoperative pain, as well as relatively high satisfaction for incision in patients.