广西医学

目的探讨柴胡疏肝汤治疗癫痫大鼠的效果及其对miR⁃34a表达的影响。方法将42只大鼠随机分为癫痫组（n=15）、柴胡疏肝汤组（n=12）及空白组（n=15）。除空白组外，另两组采用腹腔注射氯化锂-盐酸匹罗卡品法构建癫痫大鼠模型。造模结束后，给予柴胡疏肝汤组大鼠灌胃2 mL/100 g柴胡疏肝汤，空白组、癫痫组给予等量生理盐水灌胃，均为1次/d，持续灌胃3周。造模结束后，采用microRNA微阵列芯片检测癫痫大鼠模型海马组织差异表达的miRNA。干预期间观察记录3组大鼠的癫痫发作情况。最后一次灌胃结束后采用实时荧光定量PCR检测3组大鼠海马组织miR⁃34a表达水平。结果 MicroRNA微阵列芯片测序分析从癫痫大鼠海马组织中共筛选出40个差异表达的miRNA，其中miR⁃34a在癫痫大鼠模型海马组织中呈高表达。与癫痫组比较，柴胡疏肝汤组大鼠癫痫发作次数、发作持续时间减少（P<0.05）。与空白组比较，癫痫组、柴胡疏肝汤组大鼠模型海马组织中miR⁃34a的表达水平升高（P<0.05）；与癫痫组比较，柴胡疏肝汤组大鼠模型海马组织中miR⁃34a的表达水平降低（P<0.05）。结论柴胡疏肝汤可能通过抑制癫痫大鼠模型海马组织中miR⁃34a的表达而减少癫痫发作。

Objective To explore the effect of Chaihu Shugan Decoction for the treatment of rats with epilepsy and its influence on miR⁃34a expression. Methods A total of 42 rats were randomly assigned to epilepsy group (n=15), Chaihu Shugan Decoction group (n=12), or blank group (n=15). Except for the blank group, the epilepsy rat model was established on the epilepsy group and the Chaihu Shugan Decoction group by employing the method of intraperitoneal injection of lithium chloride-pilocarpine hydrochloride. After the end of modeling, rats in the Chaihu Shugan Decoction group received intragastric administration of 2 mL/100 g Chaihu Shugan Decoction, whereas the blank group and the epilepsy group received intragastric administration of equivalent normal saline. All rats were given continuous intragastric administration once a day for 3 weeks. After the end of modeling, the microRNA microarray was used to detect miRNA of differential expression in hippocampus tissues in rats model with epilepsy. The epileptic seizure of rats in 3 groups was observed and recorded during intervention. The expression of miR-34a in hippocampus tissues of the 3 groups was detected by employing the real-time fluorescent quantitative PCR after the last time of intragastric administration. Results The analysis of MicroRNA microarray sequencing revealed that a total of 40 miRNAs of differential expression were screened out from hippocampus tissues in rats with epilepsy, therein miR-34a was high-expressed in hippocampus tissues of rats model with epilepsy. Compared with the epilepsy group, the Chaihu Shugan Decoction group exhibited decreased frequency of epileptic seizure, and shorter seizure duration (P<0.05). Compared with the blank group, the epilepsy group and the Chaihu Shugan Decoction group yielded elevated miR-34a expression in hippocampus tissues of rats model (P<0.05). Compared with the epilepsy group, the Chaihu Shugan Decoction group interpreted decreased miR-34a expression in hippocampus tissues of rats model (P<0.05). Conclusion Chaihu Shugan Decoction may reduce epileptic seizure by inhibiting expression of miR-34a in hippocampus tissues of rats model with epilepsy.