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专家账号密码找回目的基于两样本孟德尔随机化（MR）分析探讨肠道菌群与衰弱之间的关联性。方法分别从MiBioGen数据库、IEU OpenGWAS Project数据库获取肠道菌群样本、衰弱样本的全基因组关联研究（GWAS）数据。以肠道菌群作为暴露因素，衰弱作为结局，与各类肠道菌群相关的单核苷酸多态性(SNP)作为工具变量。筛选合格的工具变量后，分别采用逆方差加权（IVW）法、加权中位数（WM）法、MR-Egger检验进行两样本MR分析。采用Cochran′s Q检验、MR-Egger截距测试、留一分析进行敏感性分析。结果拟杆菌纲(IVW法：OR＜0，P＜0.05)和拟杆菌目（IVW法：OR＜0，P＜0.05）是衰弱发生的保护因子，β变形菌纲（IVW法及WM法：OR＞0，P＜0.05）、伯克氏菌目（WM法：OR＞0，P＜0.05）、真杆菌属（Coprostanoligenes组）（IVW法及WM法：OR＞0，P＜0.05）和梭状芽孢杆菌属（Innocuum组）（IVW法：OR＞0，P＜0.05）是衰弱发生的危险因子。Cochran′s Q检验未发现异质性效应（P＞0.05），MR-Egger截距测试未发现水平多效性（P＞0.05），留一法分析结果提示，已鉴定的6个肠道菌群与衰弱之间的因果效应不受任何单个的SNP驱动。结论部分肠道菌群与衰弱之间存在因果效应，但具体机制仍需进一步研究。
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论著.生物信息技术
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更新时间：2024-02-26
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基于两样本孟德尔随机化分析的肠道菌群与衰弱的关联性研究
Association study of intestinal flora with frailty: an analysis based on two-sample Mendelian randomization
广西医学 2023第45卷23期 页码：2880-2885
作者机构：韦宜汝，在读硕士研究生，研究方向为肠道微生物与HIV感染的相关性研究。
基金信息：国家自然科学基金（82273694）；广西自然科学基金（2023GXNSFDA026036）
- 中文简介
- 英文简介
- 参考文献
ObjectiveTo explore the association of intestinal flora with frailty based on two-sample Mendelian randomization (MR) analysis.MethodsGenome-wide association study (GWAS) data of intestinal flora samples and frailty samples were obtained from the databases of MiBioGen and IEU OpenGWAS Project, respectively. Intestinal flora was used as exposure factor, frailty as outcome, and single nucleotide polymorphism (SNP) associated with various intestinal floras was used as instrumental variable. After screening qualified instrumental variable, inverse variance weighting (IVW) method, weighted median (WM) method, and MR-Egger test were used for two-sample MR analysis, respectively. The Cochran′s Q test, MR-Egger intercept test and leave-one-out analysis were used for sensitivity analysis. ResultsBacteroides (IVW method: OR＜0, P＜0.05) and Bacteroidales (IVW method: OR＜0, P＜0.05) were the protective factors for the occurrence of frailty, Betaproteobacteria (IVW and WM methods: OR＞0, P＜0.05), Burkholderiales (WM method: OR＞0, P＜0.05), Eubacterium (Coprostanoligenes group) (IVW and WM methods: OR＞0, P＜0.05) and Clostridium (Innocuum group) (IVW method: OR＞0, P＜0.05) were the risk factors for the occurrence of frailty. No heterogeneity effect was found by the Cochran′s Q test (P＞0.05), no horizontal pleiotropy was found by the MR-Egger intercept test (P＞0.05), and the results of leave-one-out analysis indicated that the causal effects of the six identified intestinal floras on frailty were not driven by any single SNP.ConclusionThere are causal effects between some intestinal floras and frailty, but the specific mechanisms still need to be further studied.
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