ObjectiveTo explore the effect of long non-coding RNA (lncRNA) deleted in lymphocytic leukemia 2 (DLEU2)on biological behavior of cervical cancerous cells, and to investigate its mechanism based on miR-21 and Wnt/β-catenin signaling pathway. MethodsCervical cancerous HeLa cells were assigned to control group, DLEU2 interfering group, DLEU2 interfering empty carrier group, DLEU2 over-expression group, or DLEU2 over-expressed empty carrier group. Except for the control group, cells of the remaining groups were transfected with corresponding plasmids. After 24 hours of transfection, cell proliferation activity, cell apoptosis and expressions of apoptosis-related proteins, cell cycle and mRNA expressions of cell cycle related factors, cell invasion number and expressions of epithelial-mesenchymal transition (EMT) related proteins, miR-21 expression, and Wnt/β-catenin protein and mRNA expressions were detected in various groups. Results(1) Compared with the control group, the DLEU2 over-expression group exhibited decreased cell proliferation activity, an increased rate of cell apoptosis, a down-regulated B cell lymphoma 2 (Bcl-2) protein expression, and up-regulated Bcl-2 associated protein X and Caspase-3 protein expressions (P＜0.05). (2) Compared with the control group, the DLEU2 over-expression group depicted decreased proportions of cells in phase G1 and phase S, and an elevated proportion of cells in phase G2, and down-regulated mRNA expressions of cell cycle related factors (P＜0.05). (3) Compared with the control group, the DLEU2 over-expression group yielded less number of cell invasions, a down-regulated expression of neural cadherin, an up-regulated expression of epithelial cadherin (P＜0.05). (4) Compared with the control group, the DLEU2 over-expression group interpreted a down-regulated miR-21 expression, and down-regulated mRNA and protein expressions of Wnt3a and β-catenin (P＜0.05). (5) Changes of the aforementioned indices in the DLEU2 interfering group were opposite to those in the DLEU2 over-expression group (P＜0.05), whereas there was no statistically significant difference between the two empty carrier groups and the control groups (P＞0.05). ConclusionDLEU2 may affect biological behaviors such as survival, death, EMT, and invasion in cervical cancerous cells through regulating miR-21 and inhibiting activity of Wnt/β-catenin signaling pathway.

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